mouse total akt antibody  (Millipore)

Journal: Cell reports

Article Title: Hippocampal astrocytes induce sex-dimorphic effects on memory

doi: 10.1016/j.celrep.2024.114278

Figure Lengend Snippet:

Article Snippet: Membranes were blocked for 1 h in 5% bovine serum albumin (BSA) (VWR #97062–904) in Tris-buffered saline prior to incubation overnight at 4°C in 3% BSA in TBS containing 0.2% Tween 20 (TBS-T) and the following primary antibodies: rabbit anti-phospho-CREB (S133) (1:1000, Cell Signaling 9198, RRID#AB_2561044) and mouse anti-total-CREB (1:250, Cell Signaling 9104S, RRID#AB_490881), rabbit anti-phospho-Akt (Ser473) (1:2500, Abcam ab81283, RRID#AB_2224551) and mouse anti-total-Akt (1:250, Cell Signaling 2920S, RRID#AB_1147620), or rabbit anti-mGluR3 (1:500, Abcam ab166608, RRID#AB_2833092) and mouse anti-γ-tubulin (1:2500, MilliporeSigma T5326, RRID#AB_532292).

Techniques: Recombinant, Plasmid Preparation, Bacteria, Software